SPR Technology for Affinity Analysis

The role of affinity analysis in basic research

Affinity is a parameter that describes the strength of binding between ligand and analyte molecules. Essentially, the affinity between molecules is a non-covalent force, including attractive force, hydrogen bond force, hydrophobic force and so on. The affinity can be expressed by the affinity constant KD, and its calculation formula is as follows.

[Ab], [H] and [Ab•H] represent the corresponding molar concentrations of the two interacting substances. The affinity constant actually corresponds to the equilibrium constant of the chemical reaction. The higher the affinity constant of KD and the stronger the mutual binding force of the two substances to be tested.

In many basic researches, it is necessary to analyze the affinity of ligands and analytes, and provide a basis for the binding effect and strength between them. Generally, when the binding and dissociation rate between two substances is too fast to obtain an accurate binding and dissociation rate, only the way of affinity detection can be used under this condition. Common affinity testing includes antibody affinity test, protein and small molecule peptide affinity test, protein and small molecule drug affinity test and so on. For biopharmaceutical research and development, affinity is one of the key functional attributes for judging drug efficacy. In the process of biopharmaceutical research and development, the determination of antibody affinity is of great significance. For example, the determination of antibody affinity can provide a basis for selecting monoclonal antibodies for different purposes.

Figure.1 The llustration of affinity of antigen-antibody interaction

Surface plasmon resonance (SPR) system in the process of affinity analysis

Our SPR services provide you with high-throughput, high-accuracy and high-sensitivity affinity testing, giving a strong guarantee for your experimental projects. Through our services, you can realize the affinity parallelization and automated detection of hundreds of thousands of samples in a single run. In scientific research, it is usually necessary to measure the affinity between various substances to determine their binding power, including DNA, proteins, small molecule drugs, and lipids. Creative Proteomics can provide you with accurate and effective technical consulting services before the start of your scientific research project, and customize your own testing chip after understanding your affinity testing needs. In addition, another advantage of SPR technology platform that cannot be ignored is the reusability of the chip, which can greatly reduce the cost for you. We believe that the fast, high-precision, low-cost intermolecular affinity detection is definitely what you need most in your current scientific research projects.

Fig.2 BIAchip™ in the process of affinity analysis

Choosing SPR services of Creative Proteomics, you will greatly save time and money costs owing to high-throughput intermolecular interaction detection. All services are available on a 24/7/365 basis. If you have any questions or suggestions about our services, please feel free to contact us right now.

For research use only. Not intended for any clinical use.