Protein Identification and Quantification Analysis Service

Quantitative analysis is one of the daily operations in basic experiments and is of great significance. In proteomic studies, rapid quantification of proteins is often required. Both the quantitative comparison of protein expression profiles and the analysis of protein physicochemical properties are based on accurate quantification. Accurate protein quantification is a critical step in most experiments and workflows for biochemical extraction, isolation, and analysis of proteins. Dynamic changes in intracellular proteome abundance have important implications for various life processes. For example, the occurrence and development of many diseases are often accompanied by abnormal expression of certain proteins. Quantitative proteomics is the precise quantification and identification of all proteins expressed in a genome or all proteins in a complex mixed system. In addition, with the rapid development of biological sciences, many clinically applied drugs can be reconstituted by genetic engineering. When using recombinant technology or hybridoma technology to produce protein products, the production quality management rules require operators to use effective detection methods to monitor the concentration of the produced protein products throughout the production process. Finally, the effective content of recombinant protein was calculated according to the standard curve to achieve the purpose of protein quality monitoring.

Surface plasmon resonance (SPR) in protein identification and qantification

Protein quantification is the basic analysis content of proteomics, which often requires precision and high throughput. Our SPR technology platform can fully meet the above requirements, providing you with high-throughput label-free protein quantification. The general flow of protein quantification is shown in the figure below. Proteins, as biological macromolecules, can be analyzed for concentration by direct binding assays. Firstly, a standard curve is obtained by running a protein solution of known concentration through SPR technology containing high-density ligands and then plotting the absolute response against concentration. Linear calibration curves can be used to calculate protein concentrations in unknown test samples. The standard curve method is a convenient and accurate method for protein quantification.

Fig.1 BIAchip™ in the process of protein identification and qantification

The protein concentration determination based on our SPR technology platform can involve many applications, including basic protein component concentration analysis, disease mechanism analysis caused by abnormal protein, quality monitoring of exocrine protein drugs, nutritional composition analysis, all kinds of food detection of protein content, etc. If you have protein concentration analysis needs, you can try our SPR high-throughput technology platform. SPR technology offers numerous advantages over traditional quantitative protein analysis.

• High-throughput - SPR can analyze hundreds of samples in one run, which greatly facilitates the rapid detection of a large number of samples, and facilitates the reproducibility of your protein quantification experiments.
• Accuracy - Accurate analysis of protein concentration is an essential requirement for scientific experiments. SPR not only enables accurate measurement of protein concentration, but also has significantly higher sensitivity than other protein analysis methods.
• Flexibility - All the capabilities of SPR are embodied in a small chip, which is customizable to fully meet all your protein analysis requirements.
• Low total cost - chips can be reused. In addition, multiple samples can be detected in parallel on one chip, which not only saves time, but also reduces costs.

Choosing SPR technology platform of Creative Proteomics, you will greatly save time and money costs owing to high-throughput intermolecular interaction detection. All services are available on a 24/7/365 basis. If you have any questions or suggestions about SPR, please feel free to contact us right now.

For research use only. Not intended for any clinical use.